Antioxidant

woman losing weight with palo azul

Do you want to detox your body, lose weight, prevent wrinkles and reduce your risk of cancer?

The key to all this is simple: Eliminate oxidative stress.

This can be done in 3 ways.

  1. Avoid foods or activities that cause oxidative stress
  2. Exercise
  3. Consume antioxidants

Palo Azul’s antioxidant property is among its most studied and most prominent benefits.

In fact, we reviewed over a DOZEN studies whose results concluded that Palo Azul exhibited an antioxidant effect, and many of these also found anti-inflammatory effects. Some of these studies found compounds in Palo Azul such as dihydrochalcone and methanol which induce powerful antioxidant and anti-glycation activities.

These are some of the most antioxidant foods: vegetables, fruits, nuts, seeds, fatty fish, olive oil, turmuric, cocoa and teas such as palo azul

palo azul and fruits with flavonoids

Inflammatory and oxidative foods: foods that are heavily processed and have high fructose content, trans fats and refined carbs

hamburger and fries lead to oxidative stress

What is anti-glycation you ask?

The anti-glycation property protects against AGEs (advanced glycation end products), which are harmful compounds that are considered to play an important role in the process of aging and in degenerative diseases such as diabetes, cancer, Alzheimers, and others. This is why you should be eating your vegetables, fruits, and drinking your tea every day!


If you were wondering what antioxidants are… They are compounds such as vitamins or phytochemicals like carotenoids or flavonoids which inhibit oxidative stress. They protect tissue of damage from free radicals and because of this, they prevent inflammatory responses before they can even take effect.

What is oxidative stress?

oxidative stress

Oxidative stress is an imbalance between free radicals and antioxidants in the body.

What are free radicals?

Free radicals are atoms with a single valence electron that cause damage to the body associated with diabetes, cancer, aging, atherosclerosis, Parkinson's, Alzheimer's and many other diseases.

free radicals and oxidative stress formation

This happens because electrons like to be in pairs... and for this reason, free radicals look for "free" electrons in the body. This causes damage to cells, proteins, DNA and these damaged molecules can mutate, grow tumors, and damage the DNA code. Eventually, this cellular damage causes new cells to grow with defects and it will lead to aging and degenerative diseases.

How can antioxidants help you?

They help you lose weight

woman losing weight with palo azul

One study analyzed 2754 twins and found that the group that consumed the most flavonoids:

  • Had less percentage of fat
  • Burned more fat in the abdomen

Specifically, the researchers found that the twins with the highest consumption of flavonoid-rich foods (onions, tea, apples, pears, and cocoa) “had a 3-9% lower FMR (fat mass ratio) than that of their co-twins.”

Moreover, the researchers found that the “women who ate a lot of flavonoid-rich foods had a lower percentage of body fat than women who ate less. The amount of abdominal fat also differed significantly between the two groups.”

The researchers of this study also point out that “It is important to note that abdominal fat is an important risk factor for diseases such as type 2 diabetes and heart problems.”

girl losing weight palo azul

Another study looked at 124,000 people and found that "flavonoids increase energy expenditure, decrease fat absorption and function as anti-inflammatories. The researchers in this same study found that "increased consumption of most flavonoid subclasses was associated with reduced weight gain.”

They also mentioned that the participants that consumed more flavonoids maintained their weight better and even lost some weight and they concluded the following:

"Our results suggest that choosing fruits and vegetables that are high in flavonoids, such as apples, pears, berries and peppers, can help with weight control."

You can read the full article here: Palo Azul for Weight Loss

They protect against wrinkles

woman with antioxidant skin cream

Victoria Lewis, a doctor of dermatology mentions that antioxidants are often considered the fountain of youth by skin-care professionals and experts.”

“Not only do they (antioxidants) scavenge free radicals—the number one skin-aging culprits—to promote a brighter, more even complexion, they’re also the superstars at nixing fine lines and keeping skin looking fresh.”

This is due to its ability to eliminate oxidative stress and free radicals, which are “the number one skin-aging culprits.”

This oxidative stress causes damage to our cells, and this damage eventually leads to aging and wrinkles in our skin.

Another doctor of dermatology, Dr. Mack, writes that “antioxidants protect your skin from this (oxidative stress) and help reduce pigmentation and fine lines and wrinkles.” He goes on to mention that in his opinion, “an antioxidant serum is a must for your skin-care routine.”

For this reason, many pharmaceutical and cosmetic companies supplement their skin care products with tea extracts!

You can read the full article here: Palo Azul vs Aging and Wrinkles

Reduce your risk of cancer

woman with cancer

We read two medical reviews composed of findings from 327 studies done specifically on flavonoids, and found that oxidative stress can lead to cancer, diabetes, cardiovascular disease, and aging ... For this reason, they found that antioxidants show potential to fight these diseases.

Therefore, diets rich in antioxidants decrease the cancer-promoting action of free radicals and oxidative stress. One of the studies we reviewed found that fruits and vegetables that have flavonoids have been reported as cancer chemopreventive agents and are inversely associated with cancer incidence.

palo azul vs cancer

*Here’s a very interesting fact for wine drinkers: “Moderate wine drinkers also seem to have a lower risk to develop cancer of the lung, endometrium, esophagus, stomach, and colon.”

Thank you flavonoids!

wine reduces risk of cancer

You can read the full article here: Palo Azul vs. Cancer

We also read a study that recruited 1522 breast cancer cases and 1547 frequency control subjects from June 2007 to July 2018 in Guangdong, China. The results of this study indicated that consumption of “flavonoids and most flavonoid subclasses intakes were inversely associated with breast cancer risk.”

Finally, “the critical relationship of fruit and vegetable intake and cancer prevention has been thoroughly documented. It has been suggested that major public health benefits could be achieved by substantially increasing consumption of these foods.”

In other words... oxidation in our body leads to cancer and other diseases, therefore we should consume lots of antioxidants to stay healthy.

This is why you should eat vegetables, fruit and drink palo azul tea every day!

Prepare yourself if you plan to read all the scientific research... or you can simply read our key points that we have summarized below :)

Enjoy!

... And don't forget to help your friends and family by sharing MagickTea Palo Azul with them!

book with palo azul benefits and studies

Key findings from medical studies

• 7 different studies showed that Palo Azul possesses considerable antioxidant activity, protects against oxidative injury and inhibits oxidative stress.

• 5 different studies concluded in their results that Palo Azul reduced or inhibited the formation of AGEs (advanced glycation end products).

• 4 different studies suggested that Palo Azul’s anti-glycation ability could be used to prevent diabetes complications.

• One study found that “The methanol-water extract (from Palo Azul) showed antidiabetic and anti-hyperlipidemic activities, an ability to reduce the formation of advanced glycation end products, and an antioxidant capacity in-vitro (12).


Now you see why we called it MagickTea?

Detox your body with Palo Azul and you will feel better than ever!

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Medical Studies

* Palo Azul is commonly referred to by its scientific name: Eysenhardtia polystachya / E. polystachya / E.P - Cyclolepis genistoides / C. genistoides - kidney wood - palo dulce


(Apr-Jun, 2014) Evaluation of antidiabetic, antioxidant and antiglycating activities of the Eysenhardtia polystachya

The aim of the present study was to evaluate the antidiabetic, antioxidant and antiglycation properties of Eysenhardtia polystachya (EP) bark methanol-water extract. EP showed Hdonor activity, free radical scavenging activity, metal chelating ability and lipid peroxidation. Antioxidant activity may be attributed to the presence of phenolic and flavonoid compounds. EP is an inhibitor of fluorescent AGE, methylglyoxal and the glycation of haemoglobin. In STZ-induced diabetic mice, EP reduced the blood glucose, increased serum insulin, body weight, marker enzymes of hepatic function, glycogen, HDL, GK and HK while there was reduction in the levels of triglyceride, cholesterol, TBARS, LDL and G6Pase.


Conclusion: Eysenhardtia polystachya possesses considerable antioxidant activity with reactive oxygen species (ROS) scavenging activity and demonstrated an anti-AGEs and hepatoprotective role, inhibits hyperglycemic, hyperlipidemic and oxidative stress indicating that these effects may be mediated by interacting with multiple targets operating in diabetes mellitus.

Our results in this experiment showed that E. polystachya has an antidiabetic, antihyperlipidemic, a significant ability to reduce the formation of AGEs and antioxidant activities, which are considered to play important roles in the development of diabetes complications. Therefore, this plant may have relevance in the prevention and treatment of diseases in which oxidants or free radicals or AGEs are implicated. As a result, chemical studies are now being undertaken to characterise these bioactivities.



(Sept 8, 2018) Silver Nanoparticles Synthesized Using Eysenhardtia polystachya and Assessment of the Inhibition of Glycation in Multiple Stages In Vitro and in the Zebrafish Model

The aim was to investigate the inhibitory activities on AGE formation by testing silver nanoparticles fabricated using a methanol extract of Eysenhardtia polystachya (EP–AgNPs) and characterized using various physicochemical techniques.


Conclusion: EP–AgNPs markedly reduced the formation of AGEs, Amadorin-product/fructosamine, Nε-(carboxymethyl)-lysine, amyloid cross β-structure, and protein carbonyl content in BSA-glucose system and increased total thiol-group after 4 weeks in hyperglycemic zebrafish, EP–AgNPs provided a protective effect against glycation. Data suggest that the inhibitory activity of EP–AgNPs on formation of AGEs is developed through a multiple-stage mechanism of glycation. EP–AgNPs could therefore be an antiglycation agent for prevention diabetic complications.



(June 8, 2016) Properties of Flavonoids Isolated from the Bark of Eysenhardtia polystachya and Their Effect on Oxidative Stress in Streptozotocin-Induced Diabetes Mellitus in Mice

Six new flavonoids 2′,4′-dihydroxychalcone-6′-O-β-D-glucopyranoside (1), α,3,2′,4′-tetrahydroxy-4-methoxy-dihydrochalcone-3′-C-β-glucopyranosy-6′-O-β-D-glucopyranoside (2), 7-hydroxy-5,8′-dimethoxy-6′α-L-rhamnopyranosyl-8-(3-phenyl-trans-acryloyl)-1-benzopyran-2-one (3), 6′7-dihydroxy-5,8-dimethoxy-8(3-phenyl-trans-acryloyl)-1-benzopyran-2-one (4), 9-hydroxy-3,8-dimethoxy-4-prenylpterocarpan (5), and α,4,4′-trihydroxydihydrochalcone-2′-O-β-D-glucopyranoside (6) were isolated from bark of Eysenhardtia polystachya. Results indicated that 1–5 scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (), nitric oxide radicals (), superoxide anion radical (), radical cation (), and hydrogen peroxide (H2O2) radical, and protection against H2O2 induced BSA damage was also observed. Furthermore, 1–5 showed ability to decrease the oxidative stress in H9c2 cell. The antioxidant properties of compounds 1–5 are a promising strategy for ameliorating therapeutic effects by avoiding disorders in the normal redox reactions in healthy cells which consequently could alleviate complications of diabetes.


Conclusion: Our data indicates that isolated 1–5 from the bark of Eysenhardtia polystachya have an ability to reduce oxidative stress under diabetic conditions, prevent and/or delay the onset renal, pancreatic, and hepatic damage through decreasing of lipid peroxidation, antioxidant properties, and increasing radical scavenging enzymes activity, also reduce intracellular reactive oxygen species, and they consequently could alleviate complications of diabetes. In addition, the antioxidant properties of compounds 1–5 are a promising strategy for ameliorating therapeutic effects by avoiding disorders in the normal redox reactions in healthy cells.

(May 1, 2018) Protection of silver nanoparticles using Eysenhardtia polystachya in peroxide-induced pancreatic β-Cell damage and their antidiabetic properties in zebrafish

The aim was to explore the efficacy of extract of Eysenhardtia polystachya-loaded silver nanoparticles (EP/AgNPs) on pancreatic β cells, INS-1 cells, and zebrafish as a valuable model for the study of diabetes mellitus. EP/AgNPs promote pancreatic β-cell survival, insulin secretion, enhanced hyperglycemia, and hyperlipidemia in glucose-induced diabetic zebrafish. EP/AgNPs also showed protection of the pancreatic β-cell line INS-1 against hydrogen peroxide-induced oxidative injury.

The tree Eysenhardtia polystachya (Ortega) Sarg, which belongs to the family Leguminosae, is known as “palo azul” and has been widely used as an antirheumatic and in the treatment of nephrolithiasis and bladder disorders that occur in diabetes.27 Phytochemical studies indicate that E. polystachya contains polyphenols.28 In another study, isoflavans displayed the moderate cytotoxic activity against KB cell lines.29 A methanol–water extract of the bark of E. polystachya showed antioxidant potential, hypoglycemic effects, and inhibition of the formation of advanced glycation end products (AGEs).30 The effect of flavonoids isolated from the bark of E. polystachya on oxidative stress in STZ-induced diabetes mellitus in mice was also examined.31 In other research, we determined that dihydrochalcones inhibit the formation of AGEs.32 The aim of the present study was to evaluate the effects of E. polystachya-loaded silver nanoparticles (EP/AgNPs) on glucose-induced diabetic zebrafish and peroxide-induced pancreatic INS-1 cell damage.

To produce an antidiabetic effect in zebrafish, the EE of EP must be significant. When the EP extract was characterized by UV-vis spectroscopy (Figure 1), two peaks at 284 and 325 nm, which are characteristic of the flavonoid bands I and II, respectively, were observed.41 The EP encapsulation efficiency of the obtained nanoparticles was found to be 84.8%. The synthesized AgNPs displayed an incorporation efficiency of 44.34%, showing that they were effectively loaded.

Treatment with EP/AgNPs alone or in the presence of H2O2 could ameliorate insulin sensitivity and glucose metabolism (Figure 6). EP/AgNPs promote pancreatic β-cell survival, insulin secretion-enhanced hyperglycemia, and hypolipidemia. These results confirming the effectiveness of nanoparticles in ameliorating hyperglycemia.

EP/AgNP treatment may provide beneficial effects on the function of insulin secretion through its protective effect on pancreatic β cells.48 In addition, a zebrafish model was used to confirm the antidiabetic efficacy of EP/AgNPs on experimental diabetic animals.

The findings demonstrated that diabetic fish had higher serum insulin concentrations than control zebrafish, suggesting pancreatic β-cell damage in this group, while EP/AgNPs at a dose of 100 µg/mL resulted in decreased plasma insulin levels (by 54%) compared with the glucose-induced diabetic fish group, which was similar to normoglycemic control zebrafish (Figure 8B). The results confirm the ability of EP/AgNPs to ameliorate cell viability and insulin secretion in zebrafish under high glucose concentrations. In this study, the treatments of zebrafish glucose-induced diabetes with EP/AgNPs were consistent with those reported in previous studies, confirming the effectiveness of nanoparticles in ameliorating hyperglycemia.


Conclusion: The results indicate that EP/AgNPs have good antidiabetic activity and therefore could be used to prevent the development of diabetes. The biosynthesized AgNPs from bark methanol/water extract of E. polystachya contain bioactive compounds such as chalcones, flavonoids, and dihydrochalcones, which play a determinant role in the phytofabrication of the AgNPs. EP/AgNPs promote pancreatic β-cell survival, insulin secretion-enhanced hyperglycemia, and hyperlipidemia in glucose-induced diabetic zebrafish. In addition, EP/AgNPs restore insulin secretion from INS-1 cells stimulated by H2O2, suggesting that this could result from cytoprotection against oxidative injury. These findings suggest that EP/AgNPs could be used as therapeutic nanoparticles to prevent the development of diabetes.



(Dec 1, 2011) SEPARACIÓN Y EVALUACIÓN DEL EFECTO ANTIINFLAMATORIO Y ANTIOXIDANTE DE LOS FLAVONOIDES DE Eysenhardtia polystachya (Ort.) Sarg.

Flavonoids exhibited strong antioxidant activity and other properties that are capable of inhibiting the inflammatory process by different mechanisms of action in vitro and in vivo. Recent research in our laboratory demonstrated the presence of flavonoids in Eysenhardtia polystachya (Ort.) Sarg. (sweet wood) a tree widely distributed in Mexico, as well as anti-inflammatory activity of leaves and bark after the administration of infusion and decoction at a dose of 200 mg/kg (bw) and ethanol extract at doses of 50 and 100 mg/kg (bw) in a murine model of chronic granulomatous inflammation. Therefore, the aim of this study was to separate the flavonoid-rich fraction of Eysenhardtia polystachya (Ort.) Sarg. and evaluate its anti-inflammatory and antioxidant effects.

Final and dry weights of the granulomas obtained from rats treated with fractions rich in flavonoids and indomethacin significantly decreased as compared to the control group.


Conclusion: The results of this research show that the fractions rich in flavonoids induce an anti-inflammatory effect in chronic inflammation, probably produced by its selective inhibition of COX-2



(March 28, 2019) 3-O-β-d-glucopyranosyl-α,4,2,4,6-pentahydroxy-dihydrochalcone, from Bark of Eysenhardtia polystachya Prevents Diabetic Nephropathy via Inhibiting Protein Glycation in STZ-Nicotinamide Induced Diabetic Mice

Previous studies have shown that accumulation of advanced glycation end products (AGEs) can be the cause of diabetic nephropathy (DN) in diabetic patients. Dihydrochalcone 3′-O-β-d-glucopyranosyl α,4,2′,4′,6′-pentahydroxy–dihydrochalcone (1) is a powerful antiglycation compound previously isolated from Eysenhardtia polystachya. After 5 weeks, these elevated markers of dihydrochalcone treatment (25, 50 and 100 mg/kg) were significantly (p < 0.05) attenuated. In addition, they ameliorate the indices of renal inflammation as indicated by ICAM-1 markers. The kidney and circulatory AGEs levels in diabetic mice were significantly (p < 0.05) attenuated by (1) treatment. Histological analysis of kidney tissues showed an important recovery in its structure compared with the diabetic group. It was found that the compound (1) attenuated the renal damage in diabetic mice by inhibiting AGEs formation.

In previous studies, we isolated several dihydrochalcones from the Bark of Eysenhardtia polystachya [10], which showed an efficient inhibition of fluorescent and non-fluorescent AGE formation, reduced level of fructosamine, significantly suppressed oxidation of thiols and protein carbonyl content in a BSA/glucose system; in addition, inhibited generation of MGO, and the formation of amyloid cross-β structure.


Conclusion: Our findings suggest that the treatment with dihydrochalcone protects renal function and prevents kidney injury in STZ-nicotinamide induced diabetic nephropathy, ameliorated markers of DN, as well as inflammation, HbA1C, AGE-inhibition in kidneys and circulation.

The renoprotective effect of dihydrochalcone isolated from Eysenhardtia polystachya might be associated in part to its ability to react with reactive carbonyl species and cleavage of pre-formed AGEs within the kidney by a cross- link breaker inhibiting AGEs-formation.



(Summer, 2018) Silver nanoparticles synthesized with a fraction from the bark of Eysenhardtia polystachya with high chalcone and dihydrochalcone content effectively inhibit oxidative stress in the zebrafish embryo model

In this study, we describe a simple eco-friendly approach for the synthesis of a potent, stable and benign silver nanoparticles to carry and deliver chalcones and dihydrochalcones present in a methanol extract of Eysenhardtia polystachya (EP). This study also demonstrated the biocompatibility of the nanoparticles when tested in RAW264.7 cells and its protective efficacy against oxidative stress induced by the exposure of zebrafish embryos to high glucose concentrations. Treatment with EP/AgNPs increased the activity of anti-stress biomarkers such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and total soluble protein. Exposure of the embryos to EP/AgNPs significantly (P<0.05) suppressed the formation of malondialdehyde (MDA) and lipid oxidation (LPO).


Conclusion: EP/AgNPs synthesized from E. polystachya extract provide an effective defense against oxidative stress in zebrafish embryos.



(Winter 2018) The Ethanolic Extract of Eysenhardtia polystachya (Ort.) Sarg. Bark and Its Fractions Delay the Progression of Rheumatoid Arthritis and Show Antinociceptive Activity in Murine Models

Eysenhardtia polystachya is widely used in folk medicine as an anti-rheumatic and analgesic agent, but no systematic study of its effects on several markers associated with rheumatoid arthritis and its ethnomedical use as analgesic agent has been performed. We evaluated the anti-arthritic and antinociceptive properties of an ethanolic extract of E. polystachya (EE) bark and its rich-flavonoids fractions in murine models. Many plant constituents, including flavonoids, have proven effective against arthritis by reducing cartilage degradation, diminishing leukocyte infiltration in the synovial space, decreasing serum cytokine levels, and other mechanisms (9). Phytochemical studies indicate that E. polystachya contains polyphenols, and previous chemical examination of this species led to the isolation and structural elucidation of several flavonoids (11, 12). The methanol-water extract showed antidiabetic and anti-hyperlipidemic activities, an ability to reduce the formation of advanced glycation end products, and an antioxidant capacity in-vitro (12).


Conclusion: EE and its rich-flavonoids fractions inhibited secondary inflammatory reactions, diminished the specific histopathological alterations in the joint capsule and reduced the serum concentrations of the pro-inflammatory cytokines IL-6, TNF-α, and GM-CSF in arthritic rats. EE also reduced the number of writhes produced by acetic acid and increased the response time on the hot plate for mice. Our findings support the use of Eysenhardtia polystachya bark for the treatment of rheumatoid arthritis and pain management.

In conclusion, the ethanolic extract of Eysenhardtia polystachya bark and its rich-flavonoids fractions inhibited secondary inflammatory reactions in arthritic rats and delayed histopathological alterations of joint capsules. Eysenhardtia polystachya also decreased the serum levels of pro-inflammatory cytokines IL-6, TNF-α, and GM-CSF and showed antinociceptive activity at the peripheral and central levels, which suggests that this plant has an effect on the cellular immune response. These findings support the use of Eysenhardtia polystachya in Mexican folk medicine for treating rheumatoid arthritis and pain management.

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